Andrographis paniculata is one of the most potent medicinal plants used in Indian traditional systems of medicine for the treatment of various diseases of mankind. The present study mainly aimed to estimate the content of major constituent andrographolide present in Andrographis paniculata collected from different regions of Tamilnadu and Kerala. HPTLC method was adopted for determining the content of the active constituent present in the plant using marker compound andrographolide. The HPTLC method was performed using HPTLC aluminium sheets precoated with silica gel 60 GF254 as stationary phase and ethyl acetate: n-hexane (8.5:1.5 v/v) as the mobile phase. The developed chromatogram was scanned at 233 nm using Camag scanner III. The Rf value of standard andrographolide and andrographolide in the aqueous leaf extract of Andrographis paniculata were found to be in the range of 0.28 to 0.33. The content of andrographolide was found to be high in leaves compared to other parts of the plant. Plant collected from Sirumalai hills, Dindigul, Tamilnadu and from Kuravilangad, Kerala was found to contain relatively high amount of marker compound andrographolide than other regions. Among these two regions the content of andrographolide was found to be high for the plants collected from Sirumalai hills, Dindigul region, Tamilnadu.
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